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Single Molecule Spectroscopy
by Dr. Carsten Dosche

 

In the last 15 years, single molecule spectroscopy has become an important tool in chemistry and life sciences. Various methods of superresolution microscopy are based on single molecule spectroscopy.

In contrast to other spectroscopic methods which always measure the median of a large amount of molecules, single molecule spectroscopy offers the possibility to differentiate between subpopulations of molecules. Possible applications are distance determinations in proteins, measuring the hydrodynamic size of colloids like nanoparticles or micelles and kinetics of of photo- or electrochemical reactions on the ns to ms scale.

The equipment user for single molecule spectroscopy can also be used for time resolved fluorescence spectroscopy, fluorescence anisotropy and spectroelectrochemistry. The latter is currently used in projects dealing with the formation and distribution of photo- or electrochemical reaction products.

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