Decomposition of Recal..

Decomposition of recalcitrant dissolved organic matter in coastal zones: bacterial diversity and catabolic pathways

(EU-Marie-Curie Individual Fellowship awarded to Dr. Veljo Kisand)

The main addressed questions are: which bacterial species consume recalcitrant organic matter in coastal zone of the German Wadden Sea; what is the biogeography of these species in coastal zone versus river basins; do any bacteria inhabiting aquatic systems possess ecto- or extracellular enzyme activity catalyzing dissolved organic matter of polyaromatic character; which are the enzymes involved in these processes.

Methodology: Bacteria decomposing allochtonous matter are "fractionated" on the bases of enrichment cultures. Preliminary characterization of total bacterial community is done by DGGE analyses. Taxonomic affiliation is determined according to present 16S rDNA methodology. Selective plates, artificial chromogenic substrates and standard test microplates are used for initial rapid screening of enzymatic properties.

Kisand, V., Simon, M. Significant decomposition of riverine humic-rich dissolved organic carbon by estuarine and marine bacteria assessed in sequential chemostat experiments, submitted.

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