Dissolved organic matter contains a suite of organic molecules that can be separated and quantified using liquid chromatography. Prominent examples are individual amino acids and carbohydrates.
Carbohydrate analysis uses a specific HPLC application called high-performance anion-exchange chromatography (HPAE), working at high pH and using strong anion exchange stationary phases. Pulsed amperometric detection (PAD) is an electrochemical detection method that has been optimized for carbohydrate analysis.
Thermo Scientific Dionex ICS-5000+: SP Single Pumps (gradient pump with degassing unit), DC Chromatography Compartment, ED Electrochemical Detector, AS-AP Autosampler.
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