Modern methods of proteogenomic research applied to environmental bacteria offer detailed insights into their molecular physiology. For best possible interpretation of corresponding data we adopt and develop new analysis methods. We further model physiological phenomena observed in chemostat-experiments serving both as a complement and source of novel hypotheses.
Estimates about biological, technical and total variation inherent to 2D DIGE analysis are important for defining the threshold of significance for protein abundance changes. Corresponding data from replicate cultures (left) were visualised with a multidimensional scaling technique (right) for both identical (A-C) and different substrate conditions (D). Details and results can be found in DOI: 10.1002/pmic.201100071
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