Leica SP8 confocal

Contacts

Scientific director

Prof. Dr. Henrik Mouritsen

Superusers

Dr. Petra Bolte

Tel. +49 441 798 3478

Apl. Prof. Dr. Karin Dedek

Tel. +49 441 798 3425

General email
 

W4 0-081

Leica SP8 confocal

Confocal: The system is a standard inverted laser scanning confocal microscope.

Excitation: The system is equipped with  3 optically pumped semiconductor lasers: 405nm, 488nm, 564nm and 1 diode laser.

Leica-SP8

Detection: Emission wavelength collection is flexible because of the filter-free spectral detection system. The system is equipped with three point detectors; two PMT detectors and one hybrid (HyD) detector. 

Objectives: 10X/0.3 dry, 20X/0.75 IMM (multi immersion: for water, glycerol, and oil), 40X/1.3 oil, 63X/1.4 oil.

Further features: The system features eg. multiple ROI selection, scan speeds up to 7 frames/s (512x512 pixels), resonant scanner (8 kHz) with scan speeds of up to 28 frames/s (512 x 512 pixels), xyz, xzy, xyzt, xzyt, and lambda scan modes (both excitation and emission).

Manual for microscope usage.

Software for data analysis can be found here Leica LAS X

 

(Stand: 21.08.2020)