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Dr. Marion Pohlner

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marion.pohlner1[at]uol.de

Postdoc

Dr. Marion Pohlner

Institut für Chemie und Biologie des Meeres (ICBM)

https://www.icbm.de/

W15-0-002 (» Adresse und Lageplan )

+49 441 798-5378  (F&P


Research interests

My main research interest is the microbial ecology of marine sediments, especially of deep-sea sediments. For this purpose, I joined two research cruises to the Pacific Ocean (SO248 and SO254) and sampled sediments for my PhD project. I´m generally very interested in the composition of benthic microbial communities and the environmental conditions shaping the community compositions. However, special focus of my work is on the Roseobacter group and its biogeographical distribution. Furthermore, I investigate members of this group regarding their metabolic capacities by genome analysis and physiological experiments. Also cultivation and isolation of new strains is a main aim of my research.

Scientific education and positions

2019 - present

Postdoc for research in CRC „Roseobacter“ (project A2) and teaching of courses in the study program „Microbiology“ at the Institute for Chemistry and Biology of the Marine Environment

2015 – 2019

PhD student in CRC „Roseobacter“, Carl von Ossietzky University of Oldenburg, Institute for Chemistry and Biology of the Marine Environment
Thesis: „Contribution of the Roseobacter group to the abundance, distribution and activity of microbial communities in marine sediments”

2014 – 2015

Scholarship in CRC „Roseobacter“, Carl von Ossietzky University of Oldenburg, Institute for Chemistry and Biology of the Marine Environment
Project: „Anaerobic growth of members of the Roseobacter group“

2011 –2014

MSc in Microbiology, Carl von Ossietzky University of Oldenburg, Institute for Chemistry and Biology of the Marine Environment
Master thesis: “Microbial response to acetate amendment in sediment incubations”

2008 –2011

BSc in Biology, University of Bayreuth
Bachelor thesis: “Microbial activity along a vertical pine forest soil profile”

Research Cruises / Experiences abroad

Expedition SO254 (RV Sonne, February 2017)
Functional diversity of bacterial communities and the metabolome in the water column, sediment and in sponges in the southwest Pacific around New Zealand - Microbial abundance, diversity and activity in Pacific Deep Sea sediments

Expedition SO248 (RV Sonne, May 2016)
Ductional diversity of bacterial communities and the geometabolome in the central and north Pacific - Microbial abundance, diversity and activity in Pacific Deep Sea sediments

Lab visit at Texas A&M University - Corpus Christi and University of Houston - Clear Lake, Texas, USA (03/2015); cooperation partners: B. Kiel Reese and H. Mills

Workshop “Genomics in Environmental Microbiology” at Aarhus University (10/2017); led by Ian Marshall and Lars Schreiber

Conferences

VAAM 2019, Mainz, Germany: Pohlner M, Degenhardt J, Wemheuer B, Schnetger B, Engelen B „Abundance and diversity of Bacteria and Archaea within deep-sea sediments along a Pacific transect from New Zealand to Alaska” (Presentation)

ISME 2018, Leipzig, Germany: Pohlner M, Kiel Reese B, Dlugosch L, Wemheuer B, Mills H, Cypionka H, Engelen B „The majority of Roseobacter group members in marine sediments belong to uncultured genera: an approach to define the unknowns” (Poster)

VAAM 2018, Wolfsburg, Germany: Pohlner M, Degenhardt J, von Hoyningen-Huene A, Wemheuer B, Engelen B „The distribution of benthic Roseobacter group members in the Pacific is shaped by nutrient availability at the seafloor and productivity in the water column” (Poster)

ISME 2016, Montreal, Canada: Kanukollu S, Pohlner M, Wemheuer B, Ghanem N, Herber J, Cypionka H, Engelen B „The Roseobacter group in marine sediments – abundance, diversity and metabolic potential“ (Poster)

VAAM 2016, Jena, Germany: Pohlner M, Kiel Reese B, Mills H, Cypionka H, Engelen B “Defining unknown members of the Roseobacter group in marine sediments” (Poster)

VAAM 2015, Marburg, Germany: Pohlner M, Kanukollu S, Cypionka H, Engelen B “Identification of metabolically active microbial communities in sediments by two independent RNA-based in vivo labeling techniques” (Poster)

Publications

Pohlner M, Dlugosch L, Wemheuer B, Mills H, Engelen B, Reese BK (2019) The Majority of Active Rhodobacteraceae in Marine Sediments Belong to Uncultured Genera: A Molecular Approach to Link Their Distribution to Environmental Conditions. Front Microbiol 10:659, doi.org/10.3389/fmicb.2019.00659.

Wemheuer F, von Hoyningen-Huene AJE, Pohlner M, Degenhardt J, Engelen B, Daniel R, Wemheuer B (2019) Primary Production in the Water Column as Major Structuring Element of the Biogeographical Distribution and Function of Archaea in Deep-Sea Sediments of the Central Pacific Ocean. Archaea, Article ID 3717239, doi.org/10.1155/2019/3717239.

Ziesche L, Wolter L, Wang H, Brinkhoff T, Pohlner M, Engelen B, Wagner-Döbler I, Schulz S (2019) An Unprecedented Medium-Chain Diunsaturated N-acylhomoserine Lactone from Marine Roseobacter Group Bacteria. Mar. Drugs 2019, 17:1, doi.org/10.3390/md17010020.

Pohlner M, Degenhardt J, von Hoyningen-Huene A, Wemheuer B, Erlmann N, Schnetger B, Engelen B (2017) The biogeographical distribution of benthic Roseobacter group members along a Pacific transect is linked to oceanic provinces with comparable nutrient composition. Front Microbiol 8: 2550, https://doi.org/10.3389/fmicb.2017.02550.

Pohlner M, Marshall I, Schreiber L, Cypionka H, Engelen B (2017) Draft genome sequence of Pseudoruegeria sp. SK021, a representative of the marine Roseobacter group, isolated from North Sea sediment. Genome A 5:e00541-17, https://doi.org/10.1128/genomeA.00541-17.

Harig T, Schlawis C, Ziesche L, Pohlner M, Engelen B, Schulz S (2017) Nitrogen-containing volatiles from marine Salinispora pacifica and Roseobacter-group bacteria. J Nat Prod, https://doi.org/10.1021/acs.jnatprod.7b00789.

Kanukollu S, Voget S, Pohlner M, Vandieken V, Petersen J, Kyrpides NC, Woyke T, Shapiro N, Göker M, Klenk HP, Cypionka H, Engelen B (2016) Genome sequence of Shimia sp. SK013, a representative of the Roseobacter group isolated from marine sediment. SIGS 11:1-10, https://doi.org/10.1186/s40793-016-0143-0.

 

Applied methods

Analysis of microbial diversity by Illumina-sequencing and DGGE of 16S rRNA genes and transcripts, quantification of microorganisms and physiological groups by qPCR, cultivation of aerobic and anaerobic microorganisms, activity measurements (exoenzymes, sulfate reduction rates), stable-isotope-probing

Teaching

ICitiBM-Webmasteobjr (sibet.rjxvduiexgginger@uoywlpl.de) (Stand: 23.03.2020)