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Prof. Dr. Karl-Wilhelm Koch
Tel.: +49-(0)441-798-3640
Office: W4 1-137
E-mail: kagla66rl.w.koch@uol.devl

<br 888bd/> (irencope.we5polittebl+b@uol.demzeegnbb)

The following methods are applied in the AG Biochemistry:

  • Isolation of rod cells from bovine retinae
  • Subfractionation of rod cells (outer plexiform layer, lipid rafts etc.)
  • Purification of native retinal proteins by chromatographic procedures
  • Heterologous expression of proteins in E. coli or in eucaryotic cell culture systems
  • Mutagenesis strategies
  • Purification of heterologously expressed proteins
  • Fluorescence labelling of proteins
  • HPLC-Analysis
  • Enzymatic assays (guanylate cyclases, phosphodiesterases, kinases), 45Ca2+-binding studies
  • Electrophoresis, Immunoblotting, Immunoprecipitation
  • Surface Plasmon Resonance (SPR) Spectroscopy
  • Fluorescence Spectroscopy
  • Isothermal Titration Calorimetry (ITC)
  • Click chemistry
  • steady state fluorescence
  • Backscattering Interferometry (BSI) 
Kristinfszng Tiet3hudzje (kristin.tmk1ietfzkeje@uol.de) (Changed: 2020-01-23)