Methods
Contact
Prof. Dr. Karl-Wilhelm Koch
Tel.: +49-(0)441-798-3640
Office: W4 1-137
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Methods
The following methods are applied in the AG Biochemistry:
- Isolation of rod cells from bovine retinae
- Subfractionation of rod cells (outer plexiform layer, lipid rafts etc.)
- Purification of native retinal proteins by chromatographic procedures
- Heterologous expression of proteins in E. coli or in eucaryotic cell culture systems
- Mutagenesis strategies
- Purification of heterologously expressed proteins
- Fluorescence labelling of proteins
- HPLC-Analysis
- Enzymatic assays (guanylate cyclases, phosphodiesterases, kinases), 45Ca2+-binding studies
- Electrophoresis, Immunoblotting, Immunoprecipitation
- Surface Plasmon Resonance (SPR) Spectroscopy
- Fluorescence Spectroscopy
- Isothermal Titration Calorimetry (ITC)
- Click chemistry
- steady state fluorescence
- Backscattering Interferometry (BSI)