Immunohistochemistry: double- and triple-labelling with differently coloured fluorescent markers, confocal microscopy (right example)
Dye or lesion marking of recording sites (left example)
Recording of evoked potentials with, e.g., subcutaneous metal electrodes
Recording of single-unit action potentials with glass microelectrodes
RT-PCR on developmental cDNA libraries from chicken embryos
Cloning of expression vectors
Production of retrovirus stocks in cell culture
Virus-mediated long-term and stable gene misexpression in chicken embryos
Design and application of short hairpin (sh)RNA interference
Expression of light-activated proteins (green label in the image) in neurones of the barn owl's brain. The foreign gene was introduced by a virus, injected into the brain some weeks earlier.
We are currently optimizing the light stimulation, via a fine glass fibre (gray in the image) that is connected to a laser at the other end. Neural acitivity is simultaneously registered by an electrode (black).
Stay tuned .....