Contact

Prof. Dr. Christiane Richter-Landsberg

E-Mail: christiane.richter.landsberg@uol.de

Methods

Methods

Cell culture techniques

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Clonal cell lines
  • OLN-93, C6 cells, PC12 cells, 3T3 cells

Primary cultures
  • Neuronal cultures
    (rat cerebral neurons, dorsal root ganglion neurons)

  • Glia cell cultures
    (oligodendrocytes, astrocytes, microglia)

Cell viability determination

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  • MTT-assay, Neutralred-assay

  • Trypan Blue staining, LDH-assay

  • Nuclear staining, TUNEL staining

  • Mitochondrial integrity, Proteasome activity

Microscopy

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  • phase contrast microscopy

  • Hoffman modulation contrast Imaging

  • Fluorescence microscopy

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  • Confocal laser microscopy

  • Laser capture microscopy (followed by PCR analysis)

  • In vivo imaging

Protein analysis

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  • SDS-Polyacrylamide gelelectrophoresis

  • Immunoblot analysis

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  • Immunoprecipitation

  • Microtubule binding assay

  • Solubility assay 

Molecular Biology

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  • Cell transfection

  • Small interfering RNA

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  • RT-PCR analysis


(Changed: 2021-04-30)