Clonal cell lines

• OLN-93, C6 cells, PC12 cells, 3T3 cells

Primary cultures

• Neuronal cultures
  (rat cerebral neurons, dorsal root ganglion neurons)

• Glia cell cultures
  (oligodendrocytes, astrocytes, microglia)

• MTT-assay, Neutralred-assay

• Trypan Blue staining, LDH-assay

• Nuclear staining, TUNEL staining

• Mitochondrial integrity, Proteasome activity

• phase contrast microscopy
  

• Hoffman modulation contrast Imaging
  

• Fluorescence microscopy

• Confocal laser microscopy
  

• Laser capture microscopy (followed by PCR analysis)
  

• In vivo imaging

• SDS-Polyacrylamide gelelectrophoresis

• Immunoblot analysis

• Immunoprecipitation

• Microtubule binding assay

• Solubility assay 

• Cell transfection
  

• Small interfering RNA

• RT-PCR analysis