Methods
Team leader
Address
Methods
Methods
Cell Culture:
Permanent cell lines
Molecular genetic techniques:
PCR, quantitative real-time PCR, RNA in situ hybridization, directed mutagenesis, cloning
Microscopy:
Immunocytochemistry, confocal microscopy, virtual slide scanning microscopy, laser dissection microscopy, STED-microscopy
Protein biochemical methods:
Protein gel electrophoresis, immunoblotting, subcellular fractionation, co- immunoprecipitation, GST/His-Tag Pull Down, cell surface expression analysis, Tl+ and 86Rb+ based activity measurements, protein evolution and structural analysis
