Experimental Bachelor- and Master theses are offered in one of the following topics:
Analysis of mouse models for deafness
Deafness is the most common sensory disorder in humans. In our group, we investigate the functional consequences of deafness on the level of the inner ear and the auditory brainstem in several transgenic mouse models. Experimental Bachelor and Master theses will investigate the role of deafness genes in development and maturation of the auditory pathway. To this end, anatomical studies, immunohistochemistry, protein biochemistry and PCR-based methods are performed.
Evolution of the auditory system
Whole genome sequences of an ever-increasing number of organisms, high-throughput sequencing methods as well as CrispR/Cas provide unprecedented possibilities to compare gene expression across species and to identify gene function. Individual research projects at Bachelor- and Master levels will deal with cloning and expression analyses of genes in different vertebrates such as frogs, birds, and mammals. In addition, these genes will be genetically manipulated in cell culture and organisms to identify the respective function.
Functional, biochemical, and molecular analyses of KCC2
The potassium chloride cotransporter 2 (KCC2) plays a central role in synaptic inhibition. Its dysfunction is associated with epileptic seizures, autism, schizophrenia, brain traumata, and neuropathic pain. During experimental Bachelor and Master theses, the operating mode and posttranslational modifications of KCC2 will be investigated using site-directed mutagenesis, cell culture, immunoblotting, co-immunoprecipitation, GST/His-tag pull down, cell surface expression analyses, immunocytochemistry, and thallium based flux measurements.
Successful participation of the module bio290 is required to conduct independent research modules on the Bachelor level.
Furthermore, students with interest in either a Bachelor- or Master research project should provide a short motivation letter.
Dr. Maike Claußen