The surface of diatoms represents an astonishingly diverse habitat for bacteria. A team led by biologist Meinhard Simon has now made the preferences of different species visible for the first time.
Unicellular algae are an attractive and surprisingly diverse habitat for marine bacteria. A research team led by microbiologist Professor Dr. Meinhard Simon from the University of Oldenburg has now shown for the first time how different species of bacteria tend to colonise different parts of a common microscopic diatom from the North Sea. The team presents the results of its study in the current issue of the Journal of Phycology, shedding light on the complex interactions between algae and bacteria which are of fundamental importance for material cycles and food webs in marine environments.
Diatoms are an important type of phytoplankton – the mostly microscopic, single-celled algae that live in the world's oceans. Enclosed in a solid silicate shell, diatoms produce about a fifth of the oxygen in the Earth's atmosphere and also convert carbon dioxide from the air into biomass during photosynthesis, binding more CO2 than the tropical rainforests, which is why they play a key role in the carbon cycle and for the climate. "Diatoms live in close association with bacteria, yet little is known about many aspects of their interactions," Simon explained. The biologist and his team have now taken a closer look at these microscopic interactions which are of vital importance for the ecology and biochemistry of the oceans.
Diatoms colonised by bacteria
The Oldenburg team led by Professor Simon, Dr Sara Billerbeck and doctoral candidate Tran Quoc Den selected a commonly occurring diatom called Thalassiosira rotula and used various microscopy methods to conduct a detailed analysis of its colonisation by bacteria. Dr Thomas Neu from the Helmholtz Centre for Environmental Research in Magdeburg was also involved in the study.
Using fluorescent dyes, the researchers were able to demonstrate that the surface of this alga displays distinct microscale biochemical variations. They used lectins – complex biochemical compounds that bind very specifically to certain protein-carbohydrate complexes – to mark the different areas on the surface of the alga, stain them, and with the help of a special microscope make them visible as 3D images. The scientists also found out that different species of bacteria specialise in colonising different areas of the algae. The observed bacteria belonged mainly to the Roseobacter group and the Flavobacteria strain.
"The cell surface of diatoms is surprisingly diverse in its structure. We hadn't expected the colonisation by the different bacterial species to be so finely tuned to this structure," Simon explained. The team also observed that certain bacterial species are more commonly found on fine hairs or "threads" on the surface of the algae. "The colonisation patterns reflect the highly diverse metabolic properties of different bacterial species and their abilities to colonise surfaces," said Simon, summarising the team's findings.
Algae and certain bacteria have a relationship similar to flowers and bees
These results are also interesting because they provide an insight into the diatoms' microenvironment. Known as the "phycosphere", this environment is rich in organic substances excreted by the algal cells. "Previous studies have shown that the algae use this to attract certain bacteria that produce substances which are vital for their survival, for example vitamins – similar to the way plants attract bees with their flowers," Simon explained. "Building on these new findings, future studies could examine in greater detail the precise function of the different bacterial species in the phycosphere," he added.
The current study was conducted as part of the Transregional Collaborative Research Centre (CRC) Roseobacter, which is funded by the German Research Foundation (DFG). In the 13 years since the establishment of the CRC, more than 60 researchers from Oldenburg, Braunschweig, Göttingen and Bonn have studied the bacteria of the Roseobacter group. These bacteria occur in all marine environments – from the tropics to the polar seas and from the surface layer to the deep sea. The researchers have discovered many new strains and described the interactions of representatives of this group with other microorganisms and their distribution and functional biogeography in the oceans around the world for the first time. In total, more than 250 published scientific articles are based on research conducted in the context of the CRC Roseobacter.
