Contact
Dr. Bahareh Dabirmanesh
Postdoctoral researcher (guest scientist)
Professional experience
Since 2024 PostDoc biochemistry, SFB 1372, project Sig06 (founded by DFG)
Since 2013 Academic member at Faculty of Biological Sciences, Department of Biochemistry, Tarbiat Modares University (Tehran)
2009-2013 Doctoral candidate in biochemistry at Tarbiat Modares University (Tehran)
Education
2004-2007 MSc: Biochemistry at Alzahra University (Tehran)
2000-2004 BSc: Cellular & Molecular Biology at University of Tehran (Tehran)
Publications
https://scholar.google.com/citations?user=InzhK08AAAAJ&hl=en
Honours and awards
Young researcher Award in the field of Biotechnology 2018 (from Vice presidency for Science & Technology)
Shahid Tehrani Moghaddam Award 2012
Became a member of National Elite Foundation
Books
The Hidden World of Protein Aggregation. Progress in Molecular Biology and Translational Science. Editors: David B. Teplow, Bahareh Dabirmanesh, Vladimir N. Uversky. Hardback ISBN: 9780443293405, eBook ISBN: 9780443293412
Principle of Chromatography (in Persian): Compiled by: Bahareh Dabirmanesh, Ensieh Khalkhal and edited by Farideh Ghazi
Book chapters
Recombinant laccase production: Escherichia coli, Pichia pastoris, and filamentous fungi as microbial factories, Fatemeh Aziziyan, Farnoosh Farzam, Bahareh Dabirmanesh, Khosro Khajeh In: Bacterial Laccases, Engineering, Immobilization, Heterologous production, and Industrial applications, Edited by Deepi Yadav and Tukayi Kudanga, 2024, Progress in Biochemistry and Biotechnology
Machine learning in surface plasmon resonance for environmental monitoring, Masoud Mohseni-Dargah, Zahra Falahati, Bahareh Dabirmanesh, Parisa Nasrollahi, Khosro Khajeh, In: Artificial Intelligence and Data Science in Environmental sensing- Cognitive Data Science in Sustainable Computing, Edited by Mohsen Asadnia, Amir Razmjou, Amin Beheshti, 2022, Cognitive Data Science in Sustainable Computing
Commercialisation of research
Commercializing Growth factors (bFGF, EGF, BDNF), Gattex and drug enzyme such as carboxypeptidase G2 in Ecoli and five lysosomal enzymes (Fabrazyme, Cerezyme, Myozyme, Naglazyme and Aldurazyme) in CHO cell line in a knowledge-based company named Protein Fanavar Pajouh.
Laboratory skills
Microbiological, Biochemical and Molecular Biology skills
- Culturing (including fermentation)
- Protein purification (FPLC (Q-sepharose, DEAE-Sepharose, Phenyl-Sepharose, Sephadex-G100 and Sephacryl S200, Ni-NTA), HPLC (C18, Eurokat Ag)
- Cloning
- Mutagenesis (site directed and site saturation mutagenesis)
- PCR
- RT-PCR
- Real Time PCR
- Agarose Electrophoresis
- SDS-PAGE, PAGE and Two Dimensional Gel Electrophoresis
- Silver Nitrate and Coomassie Blue Staining
- Western Blotting
- ELISA
- Concentrating and Exchanging buffer (TFF, Centricon, Salting out and Dialysis)
- Granulation Methods (Fluid Bed & High Sheer Methods)
- Fluorescence and UV-Visible spectrophotometry
- Circular Dichroism (CD)
- Isothermal Calorimetry (ITC)
- Atomic Absorption
- Differential Scanning Calorimetry (DSC)
- Dynamic Light Scattering (DLS)
- Surface Plasmon Resonance (SPR)
- Flow Cytometry
Cell biology skills
- Cell culturing
- MTT assays
- Wound healing assay
- Optical microscopy (phase contrast and fluorescence microscopy )
- Designing vectors and transfection
- Stable cell pool/single
Bioinformatics & databases
- PyMol
- SPDBV
- YASARA
- Rasmol
- Modeller
- HADDOCK
- AutoDock Vina
- ClusPro
- I-Tasser
- Robetta
- Alphafold
- Expasy
- Blast
- Brenda
- Enrich r
- KEGG pathway database
- STRING