Methods / Instruments
- Sampling (on board), filtration, fixation and storage of samples.
- Classical chemical methods for the analysis of inorganic nutrients and elements.
- High sensitivity methods for substrate analyses (HPLC, CHN, TOC analysis).
- Fractionation of molecular size classes of DOM and specific dissolved substrates by ultrafiltration.
- Methods to study the occurrence of aggregates in situ and their formation experimentally (under water photography, rolling tanks).
- Micro- and mesocosms studies.
- Microscopy and image analysis.
- Flow cytometry and cell sorting.
- Classical microbiological methods to isolate, culture and physiologically characterize bacteria.
- Molecular methods to identify bacteria and the composition of microbial communities (Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA fragments, fluorescence in situ hybridization, RT-PCR, quantitative real time PCR, cloning, sequencing of 16S rDNA genes, genomics, metagenomics).
- Light and epifluorescence microscopes
- HPLC (amino acid, monosaccharide analysis)
- CHN analysis
- Real Time
- Electrophoresis (DGGE, agarose)
- Gel documentation
- DNA sequencer
- Flow cytometer (FACS ARIAIII and C6)
- Incubation of viruses, bacteria and algal species
- Rolling tanks for the experimental simulation of tidal cycles
- Photographic under water documentation and image analysis of suspended aggregates