Core Facility Fluorescence Microscopy

Contacts

Dr Petra Bolte

Dr Levent Gütay

W4 0-081 (Huygens)

W4-0-072 (Amira)

W34-0-008 (Arivis)

Image analysis workstations

We offer access to 3 powerful windows workstations for image analysis.

The first computer runs the software package Huygens Essential by Scientific Volume Imaging (SVI), which allows to correct optical blurring, noise, spherical aberration and many others by 3D deconvolution of confocal data.

The software contains options for Widefield, Confocal and STED deconvolution.

Additional features included in the licence: Full File-readers Option, PSF Distiller (point spread function), Object Stabilizer, Deconvolution & Batch Express, Hot Pixel Remover, Twin-Slicer, Ortho-Slicer, MIP-Renderer, SFP Renderer.

This PC is part of the core facility and can be booked via the online booking calendar.

 

The following PCs belong to affiliated groups. Access is available for core facility users.

On the second computer the software Amira (Thermofisher) is installed. Amira is a software tool used for 3D and 4 D data reconstruction, visualisation and image analysis.

The third PC contains Arivis and a very large RAM memory for fast and convenient image analysis.

 

STED and confocal deconvolved

Figure 1 Comparison of confocal and STED imaging and images processed by Huygens deconvolution program. Imaging done by Isabel Gross. The image present staining of a postsynaptic protein in dendrites of the mouse brain (Oregon green).

(Changed: 11 Feb 2026)  Kurz-URL:Shortlink: https://uol.de/p75446en
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