Methods and devices
Methods and devices
Molecular biology
Devices | Techniques/Methods |
Eppendorf Mastercycler nexus Gradient | PCR |
BioRad Chemidoc MP | Western blot, gel electrophoresis |
Heratherm IMP 180 cooled incubator with internal socket | Temperature range: +5 - +70°C, immunofixations |
Eppendorf Bio Spectrometer basic | Absorbance measurements: Proteins, DNA; RNA |
Ultra Turrax IKA Disperser T10 standard | homogenization and grinding of tissue |
Eppendorf Incubation Shaker : New Brunswick Innova 40 | e.g. for incubation of E. Coli |
Elga Purelab Flex 3 ultrapure water system | Production of ultrapure water type I |
Lipid biochemistry
Devices
CAMAG TLC Sampler 4 CAMAG TLC Visualizer CAMAG TLC Scanner CAMAG Derivatizer | Techniques/Methods
HPTLC of lipids |
Nitrogen chamber | Lyophilisation of lipid samples |
Protein biochemistry / histology
Devices |
Protein fractionation |
ISMATEC IPC Pefusion Pump | Perfusion |
BioRad Trans-Blot Semi-Dry Transfer Cell BioRad Power Pac Basic | Western blot, SDS page, tank blot, semi-dryblot |
Leica VT 1000 S Vibratome | Tissue sections |
Bandelin SonoPlus | Cell lysis |
BioTek Synergy 2 | ELISA fluorescence and absorbance measurement |
Equipment room
Appliances New Brunswick Innova C585 Ultra Low Temperature Freezer -80°C | Techniques/Methods |
Taylor Wharton XL-70 | Liquid nitrogen storage and transport containers |
Drying cabinet Heratherm OMH 60 | 50-330°C |
Drying oven Heratherm OGS 60 | 25-250°C |
S2- Steam steriliser Hp-Med Varioklav 75S | Sterilisation of liquids, instruments, S1- and S2-waste |
Taylor Wharton LS-3000 nitrogen storage tank |
Cell culture
Devices 2x New Brunswick CO2 Incubator Galaxy 170S | Techniques / Methods Cultivation of prim. Neurons, astrocytes, HEK cells, Hap1 cells |
Thermo Scientific Safe 2020 safety cabinet |
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Thermo Scientific Heraguard ECO 1.2 Horizontal Laminar Flow Workbench | |
Leica S9D stereo microscope with transmitted light base TL3000ST | Preparation |
Olympus CKX53 : inverse phase contrast technology | Microscopy of cell culture samples |
IncuCyte S3 _ Live-Cell Imaging and Analysis
Objectives: 4x, 10x, 20x, air
Excitation/emission: 440-480nm/504-544nm (Green) and 565-605nm/625-705nm (Red).
Temperature regime: 0-42C.
CO2 Incubator
The image analysis software provided by IncuCyte is also available for the users.
Live-Cell Analysis System Applications: Software Modules
- Basic Analyser (Stem Cell Monitoring and Reprogramming, Cell Culture QC, Dilution cloning, Transfecion Efficiency, Reporter Genes, Proliferation, Apoptosis, Cytotoxity, Immune Cell Clustering, Antibody Internalisation, Immune Cell Killing, Phagocytosis)
- Neuro Track (Neurite Analysis)
Incucyte Vessel Trays available for: 35 mm dishes, T25 flasks, 6-/12-/24-/96-well plates
Olympus IX 83 inverted fluorescence microscope
Light source:
Cool LED pE-4000 (4-channel high-specification LED system with 16 LEDS, 365-770nm).
Lenses:
- UPlanFLN 4x/0.13 PhL
- UPlanSApo 10x/0.4
- UPlanSApo 20x/0.75
- UPlanSApo 40x/0.95
- UPlanSApo 60x/1.35 Oil
- UPlanSApo 100x/1.4 Oil
Filter modules:
- U-F39002 AT-FITC (Acridine Orange, Alexa Fluor488, eGFP, Fluo 4, FITC, Oregon Green 488)
- U-F39003 AT-EYFP N/C (eYFP)
- U-F39004 AT-CY3 (Alexa Fluor 546, Alexa Fluor 555, Cy3, Dil, TRITC)
- U-F39007 AT-CY5/ALFL647 (Alexa Fluor 647, APC, Cy5, TO PRO 3)
- IX3-FDICT (differential interference contrast, DIC)
- U-FF (DAPI)
Leica DMi8: inverted partially motorised research microscope with Ca imaging equipment
Objectives:
HC PL FLUOTAR L 20x/0.40 Dry (LMD; PH1)
HC PL FLUOTAR L 40x/0.60 Dry
HC PL FLUOTAR 40x/1.30 Oil (Fura)
HC PL APO 63x/1.40 Oil
HC FLUOTAR 100x/1.30 Oil (Fura)
Olympus BX43F Upright Transmitted Light Microscope with Digital High Performance Microscope Camera for Scanning
Light source:
True Colour LED (colour rendering index (>96), intensity equivalent to 30 W halogen).
Objectives:
6x revolving nosepiece with
- PLCN 4x/0.1
- PLCN 10x/0.25
- PLCN 20x/0.4
- PLCN 40x/0.65
- UPlanSApo 20x/0.75
Unit for digital scanning :
- Basler acA2440 camera with high frame rate in real time 5.0 MP, with Sony IMX264 sensor Frame rate: 35 fps USB 3.0
- EasyScan software, for manual high-resolution scanning of histological specimens
Co-observer module:
Dual observation tube with movable LED marker arrow
