Dr. Stefan Bruns (former Diener)

Postdoctoral Researcher
Phone.: +49-(0)441-798-3627
Room: W15 2-236

Dr. Stefan Bruns (former Diener)

Research Interest

My research focuses on the analysis and quantification of B vitamins and coenzyme A thioesters in seawater and their intra- and extracellular concentrations in bacterial cell cultures. After extraction of the samples by different techniques in the laboratory, the analytes are separated by high performance liquid chromatography (HPLC) and different mass spectrometric methods such as Selected Reaction Monitoring (SRM) or High-Resolution Full Scan (HRMS) are used for analysis. Thereby I try to draw conclusions about the origin and fate of these analytes.

Academic Career

Since Feb 2023

Postdoctoral researcher at the Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, working group Organic Geochemistry

   
Aug 2018 – Dez 2022

PhD Candidate at the Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, working group Organic Geochemistry
PhD thesis: “Quantification of B Vitamins and Coenzyme A Thioesters in Seawater and Bacteria”

   
Oct 2015 – Jul 2018

M.Sc. in Chemistry, Carl von Ossietzky University Oldenburg
Master thesis: “High performance liquid chromatography with time-of-flight mass spectrometric detection (HPLC-TOF-MS) for the analysis of coenzyme A thioesters from bacteria”

   
Oct 2012 – Sep 2015

B.Sc. in Chemistry, Carl von Ossietzky University Oldenburg
Bachelor thesis: “Studies on the α-hydroxylation of cyclic β-oxoesters.”

   

Publications

  • Bruns, S., Wienhausen, G., Scholz-Böttcher, B., Heyen, S., Wilkes, H., Method development and quantification of all B vitamins and selected biosynthetic precursors in winter and spring samples from the North Sea and de novo synthesized by Vibrio campbellii. Mar Chem 256, 104300 (2023). https://doi.org/10.1016/j.marchem.2023.104300
  • Sultana, S., Bruns, S., Wilkes, H., Simon, M., Wienhausen, G., Vitamin B12 is not shared by all marine prototrophic bacteria with their environment. ISME J (2023). doi.org/10.1038/s41396-023-01391-3
  • Bruns, S., Cakić, N., Mitschke, N., Kopke, B. J., Rabus, R., Wilkes, H., A Novel Coenzyme A Analogue in the Anaerobic, Sulfate-Reducing, Marine Bacterium Desulfobacula toluolica Tol2T. ChemBioChem, 24, e202200584 (2023). https://doi.org/10.1002/cbic.202200584
  • Bruns, S., Wienhausen, G., Scholz-Böttcher, B., Wilkes, H., Simultaneous quantification of all B vitamins and selected biosynthetic precursors in seawater and bacteria by means of different mass spectrometric approaches. Anal Bioanal Chem 414, 7839–7854 (2022). https://doi.org/10.1007/s00216-022-04317-8
  • Wienhausen, G., Bruns, S., Sultana, S., Dlugosch, L., Groon, L. A., Wilkes, H., Simon, M., The overlooked role of a biotin precursor for marine bacteria - desthiobiotin as an escape route for biotin auxotrophy. ISME J 16, 2599–2609 (2022). https://doi.org/10.1038/s41396-022-01304-w
  • Vagts, J., Kalvelage, K., Weiten, A., Buschen, R., Gutsch, J., Scheve, S., Wöhlbrand, L., Diener, S., Wilkes, H. Winklhofer, M., Rabus, R., Responsiveness of Aromatoleum aromaticum EbN1T to lignin-derived phenylpropanoids. Appl Environ Microbiol 87:e03140-20 (2021). doi.org/10.1128/AEM.03140-20
(Changed: 30 Jan 2024)  | 
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