Dr. Stefan Bruns
Postdoctoral Researcher
Phone.: +49-(0)441-798-3627
Room: W15 2-236
stefan.bruns@uol.de
Dr. Stefan Bruns
Research Interest
My research focuses on the analysis and quantification of B vitamins and coenzyme A thioesters in seawater and their intra- and extracellular concentrations in bacterial cell cultures. After extraction of the samples by different techniques in the laboratory, the analytes are separated by high performance liquid chromatography (HPLC) and different mass spectrometric methods such as Selected Reaction Monitoring (SRM) or High-Resolution Full Scan (HRMS) are used for analysis. Thereby I try to draw conclusions about the origin and fate of these analytes.
Academic Career
| Since Feb 2023 | Postdoctoral researcher at the Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, working group Organic Geochemistry |
| Aug 2018 – Dez 2022 | PhD Candidate at the Institute for Chemistry and Biology of the Marine Environment, Carl von Ossietzky University Oldenburg, working group Organic Geochemistry |
| Oct 2015 – Jul 2018 | M.Sc. in Chemistry, Carl von Ossietzky University Oldenburg |
| Oct 2012 – Sep 2015 | B.Sc. in Chemistry, Carl von Ossietzky University Oldenburg |
Publications
- Wienhausen, G., Moraru, C., Bruns, S. et al. Ligand cross-feeding resolves bacterial vitamin B12 auxotrophies. Nature 629, 886–892 (2024). https://doi.org/10.1038/s41586-024-07396-y
- Bruns, S., Wienhausen, G., Scholz-Böttcher, B., Heyen, S., Wilkes, H., Method development and quantification of all B vitamins and selected biosynthetic precursors in winter and spring samples from the North Sea and de novo synthesized by Vibrio campbellii. Mar Chem 256, 104300 (2023). https://doi.org/10.1016/j.marchem.2023.104300
- Sultana, S., Bruns, S., Wilkes, H., Simon, M., Wienhausen, G., Vitamin B12 is not shared by all marine prototrophic bacteria with their environment. ISME J (2023). doi.org/10.1038/s41396-023-01391-3
- Bruns, S., Cakić, N., Mitschke, N., Kopke, B. J., Rabus, R., Wilkes, H., A Novel Coenzyme A Analogue in the Anaerobic, Sulfate-Reducing, Marine Bacterium Desulfobacula toluolica Tol2T. ChemBioChem, 24, e202200584 (2023). https://doi.org/10.1002/cbic.202200584
- Bruns, S., Wienhausen, G., Scholz-Böttcher, B., Wilkes, H., Simultaneous quantification of all B vitamins and selected biosynthetic precursors in seawater and bacteria by means of different mass spectrometric approaches. Anal Bioanal Chem 414, 7839–7854 (2022). https://doi.org/10.1007/s00216-022-04317-8
- Wienhausen, G., Bruns, S., Sultana, S., Dlugosch, L., Groon, L. A., Wilkes, H., Simon, M., The overlooked role of a biotin precursor for marine bacteria - desthiobiotin as an escape route for biotin auxotrophy. ISME J 16, 2599–2609 (2022). https://doi.org/10.1038/s41396-022-01304-w
- Vagts, J., Kalvelage, K., Weiten, A., Buschen, R., Gutsch, J., Scheve, S., Wöhlbrand, L., Diener, S., Wilkes, H. Winklhofer, M., Rabus, R., Responsiveness of Aromatoleum aromaticum EbN1T to lignin-derived phenylpropanoids. Appl Environ Microbiol 87:e03140-20 (2021). doi.org/10.1128/AEM.03140-20