BRUKER Avance III 500
in operation since 2009
Two probes are available for this NMR spectrometer. The BBI probe is permanently fitted.
BBI probe: PA BBI 500S2 H-BB-D-05 Z
With Z-gradient
Inverse probe: ¹H coil inside – X coil outside – heteronuclei are not as sensitive,
but advantageous for ¹H and ¹H-detected 2D spectra, therefore
¹HX-HMQC and -HMBC are more sensitive than on the BBO probe.

BBO probe: PA BBO 500S2 BBF-H-D-05 Z
With Z-gradient
Unfortunately, 2D experiments no longer work with this probe. Due to its age, it cannot be repaired.
Probe: ¹H coil on the outside – XF coil on the inside → Heteronuclei are more sensitive than on the BBI probe.
With ¹H/¹⁹F switchbox

Temperature experiments are possible for both probes from -150°C to +150°C. Care must be taken to select a suitable solvent.
For temperature measurements and experiments not carried out automatically, please make an appointment.
Experiments:
Further experiments on request
| ¹H: | ¹H | |
| ¹H_para | Wide measurement range for paramagnetic nuclei; small D1 | |
| ¹H D1–120 s | → d1 (relaxation time) = 120 s | |
| ¹H¹H COSY | H–H correlation within a spin system | |
| ¹H¹H TOCSY | Total H–H correlation | |
| ¹H¹H NOESY | H–H correlation due to spatial proximity | |
| NOE diff. | Differential measurement → no automatic mode | |
| Prohomodec | Homodecoupling → no automatic mode | |
| 1H jres | Display of couplings in the second dimension → no automatic function | |
| ¹H{¹⁹F} | BBO probe only | |
| ¹H{³¹P} | ||
| ¹H{X} | Decoupling from other heteronuclei is possible | |
| PSYCHE | ¹H{¹H} | |
| DOSY | Diffusion → not automatic | |
| EXSY | Chemical exchange → no automatic mode | |
| Water suppression | → no automatic mode | |
| Solvent suppression | → no automatic mode / for non-deuterated solvents / measurement without lock / with ‘no-D’ shim | |
| ²H | Deuterium | Measurement on the lock channel |
| ⁷Li: | ⁷Li{¹H} | |
| ¹¹B: | ¹¹B | with and without background suppression |
| ¹¹B{¹H} | with and without background suppression | |
| ¹H¹¹B HMQC | ||
| ¹H¹¹B HMBC | ||
| ¹¹B¹¹B COSY | ||
| ¹¹B¹¹B COSY{¹H} | ||
| ¹³C: | ¹³C | |
| ¹³C{¹H} | ||
| ¹³C{¹H} dept135 | CH₃/CH positive – CH₂ negative | |
| ¹³C{¹H} dept90 | CH-positive | |
| ¹³C{¹H} jmod = apt | CH₃/CH positive – CH₂/C negative | |
| ¹H¹³C HMQC | not phase-sensitive | |
| ¹H¹³C HSQC | phase-sensitive (combination of HMQC and dept135) | |
| ¹H¹³C HMBC | ||
| ¹H¹³C HMBC-et | 1J suppression → no automatic mode | |
| ¹³C{¹H} inept-rd | Polarisation transfer | |
| ¹³C{¹H} zgig30 | inverse gated – integrable → weekend measurement T1 times must be known | |
| ¹³C Inadequate | no automatic mode → weekend measurement | |
| EXSY ¹³C | Chemical exchange → no automatic mode | |
| ¹³C jres | Display of couplings in the second dimension → no automatic mode | |
| ¹⁴N | ¹⁴N{¹H} | |
| ¹⁵N: | ¹H¹⁵N HMQC | Coupling constant + measurement range must be specified |
| ¹H¹⁵N HMBC | Coupling constant + measurement range must be specified | |
| ¹⁵N{¹H} inept-rd | Polarisation transfer → Coupling constant + measurement range must be specified | |
| ¹⁵N inept-pnd | Polarisation transfer → Coupling constant + measurement range must be specified | |
| ¹⁵N{¹H} dept135 | only possible if N is present as the ¹⁵N isotope | |
| ¹⁵N{¹H} zgig | Only possible if N is present as the ¹⁵N isotope | |
| ¹⁹F: | Only possible on the BBO probe | |
| ¹⁹F | ||
| ¹⁹F{¹H} 300 ppm | Measurement range width = 300 ppm | |
| ¹⁹F{¹H} 150 ppm | Measurement range width = 150 ppm / Centre of the measurement range must be specified | |
| ²⁷Al: | ²⁷Al | with and without background suppression |
| ²⁷Al{¹H} | with and without background suppression | |
| ¹H²⁷Al HMBC | ||
| ²⁹Si: | ²⁹Si | Weekend measurements – switch to inept-pnd where possible |
| ²⁹Si{¹H} zgig | inverse gated → 3h measurement time – switch to inept-rd where possible | |
| ²⁹Si{¹H}-inept-rd | Polarisation transfer | |
| ²⁹Si-inept-pnd | Polarisation transfer | |
| ¹H²⁹Si HMQC | ||
| ¹H²⁹Si HMBC | ||
| ²⁹Si Inadequate | No automatic mode → Weekend measurement | |
| ³¹P: | ³¹P | |
| ³¹P{¹H} | ||
| ³¹P{¹H} zgig | inverse gated – integrable → T1 times must be known | |
| ¹H³¹P HMQC | ||
| ¹H³¹P HMBC | ||
| ³⁵Cl: | ³⁵Cl{¹H} | |
| ⁴⁷Ti: | ⁴⁷Ti{¹H} | aring – anti-aring sequence |
| ⁷¹Ga: | ⁷¹Ga{¹H} | |
| ⁷⁵As: | ⁷⁵As{¹H} | |
| ⁷⁷Se: | ⁷⁷Se | |
| ⁷⁷Se{¹H} | ||
| ¹H⁷⁷Se HMQC | ||
| ¹H⁷⁷Se HMBC | ||
| ¹¹⁹Sn: | ¹¹⁹Sn{¹H} zgig | inverse gated |
| ¹H¹¹⁹Sn HMQC | ||
| ¹H¹¹⁹Sn HMBC | ||
| ¹¹⁹Sn{¹H} inept-rd | Polarisation transfer | |
| ¹¹⁹Sn inept-pnd | Polarisation transfer | |
| ¹²⁵Te: | ¹²⁵Te{¹H} zgig | inverse gated |
| ¹H¹²⁵Te HMBC | ||
| ¹²⁵Te{¹H} inept-rd | Polarisation transfer | |
| ¹²⁵Te inept-pnd | Polarisation transfer | |
| ¹⁹⁵Pt | ¹⁹⁵Pt{¹H} | |
| ²⁰⁷Pb: | ²⁰⁷Pb{¹H} | |