BRUKER Avance III 500
BRUKER Avance III 500
in operation since 2009
Two probes are available for this NMR spectrometer. The BBI probe is permanently fitted.
BBI probe: PA BBI 500S2 H-BB-D-05 Z
With Z-gradient
2H coil (Lock)
1H coil inside
Heteronucleus (X) coil (BB) outside
- 1H and 1H detected 2D spectra e. g. 1HX-HMQC and -HMBC are more sensitive than those at the BBO probe.
- Heteronuclei (X) are less sensitive than those at the BBO probe.
- no 19F
BBO probe: PA BBO 500S2 BBF-H-D-05 Z
Unfortunately, 2D experiments no longer work with this probe. Due to its age, it cannot be repaired. 1D experiments are still possible.
With Z-gradient
2H coil (Lock)
1H coil outside
Heteronucleus (X/19F) coil (BBF) inside
With 1H/19F switchbox
Heteronuclei (X) are more sensitive than those at the BBI probe.
Temperature experiments can be carried out on both probes within a range of -150°C up to +150°C. Care must be taken to select a suitable solvent.
For temperature measurements and experiments not carried out automatically, please make an appointment.
Experiments:
Further experiments on request
| 1H: | 1H | |
| 1H para | Wide measurement range for paramagnetic nuclei; small D1 | |
| 1H D1-120s | → D1 (relaxation delay) = 120s | |
| 1H1H COSY | HH correlation within a spin system | |
| 1H1H TOCSY | HH total correlation | |
| 1H1H NOESY | HH correlation due to spatial proximity | |
| NOE Diff | Differential measurement → no automatic mode | |
| Prohomodec | Homodecoupling → no automatic mode | |
| 1H jres | Display of couplings in the second dimension → no automatic function | |
| 1H{19F} | Only possible on the BBO probe | |
| 1H{31P} | ||
| 1H{X} | Decoupling from other heteronuclei is possible | |
| PSYCHE | 1H{1H} | |
| DOSY | Diffusion → no automatic processing | |
| EXSY | Chemical exchange → no automatic mode | |
| Water suppression | → no automatic mode | |
| Solvent suppression | → no automatic mode / for non-deuterated solvents / measurement without lock / with ‘no-D’ shim | |
| 2H | Deuterium | Measurement on the lock channel |
| 7Li | 7Li{1H} | |
| ¹¹B | 11B | with or without background suppression |
| 11B{1H} | with or without background suppression | |
| 1H11B HMQC | ||
| 1H11B HMBC | ||
| 11B11B COSY | ||
| 11B11B COSY{1H} | ||
| 13C | 13C | |
| 13C{1H} | ||
| 13C{1H} dept135 | CH3/CH-positive – CH2-negative | |
| 13C{1H} dept90 | CH-positive | |
| 13C{1H} jmod = apt | CH₃/CH positive – CH₂/C negative | |
| 1H13C HMQC | not phase-sensitive | |
| 1H13C HSQC | phase sensitive (combination of HMQC and dept135) | |
| ¹H¹³C HMBC | ||
| 1H13C HMBC-et | 1J suppression → no automatic mode | |
| 13C{1H} inept-rd | Polarisation transfer | |
| 13C{1H} zgig30 | Inverse gated – integrable → weekend measurement T1 times must be known | |
| 13C Inadequate | no automatic mode → weekend measurement | |
| EXSY 13C | Chemical exchange → no automatic mode | |
| 13C jres | Display of couplings in the second dimension → no automatic mode | |
| 14N | 14N{1H} | |
| 15N | 1H15N HMQC | Coupling constant + measurement range must be specified |
| 1H15N HMBC | Coupling constant + measurement range must be specified | |
| 15N{1H} inept-rd | Polarisation transfer → Coupling constant + measurement range must be specified | |
| 15N inept-pnd | Polarisation transfer → Coupling constant + measurement range must be specified | |
| 15N{1H} dept135 | Only possible if N is present as the 15N isotope | |
| 15N{1H} zgig | Only possible if N is present as the 15N isotope | |
| 19F | Only possible on the BBO probe | |
| 19F | ||
| 19F{1H} 300 ppm | Measurement range width = 300 ppm | |
| 19F{1H} 150 ppm | Measurement range width = 150 ppm / the centre of the measurement range must be specified | |
| 27Al | 27Al | with and without background suppression |
| 27Al{1H} | with and without background suppression | |
| 1H27Al HMBC | ||
| 29Si | 29Si | Weekend measurements – switch to inept-pnd where possible |
| 29Si{1H} zgig | inverse gated → 3-hour measurement time – switch to inept-rd if possible | |
| 29Si{1H}-inept-rd | Polarisation transfer | |
| 29Si-inept-pnd | Polarisation transfer | |
| 1H29Si HMQC | ||
| 1H29Si HMBC | ||
| 29Si Inadequate | No automatic mode → Weekend measurement | |
| 31P | 31P | |
| 31P{1H} | ||
| 31P{1H} zgig | Inverse gated – integrable → T1 times must be known | |
| 1H31P HMQC | ||
| 1H31P HMBC | ||
| 35Cl | 35Cl{1H} | |
| 47Ti | 47Ti{1H} | aring - antiringing sequence |
| 71Ga | 71Ga{1H} | |
| 75As | 75As{1H} | |
| 77Se | 77Se | |
| 77Se{1H} | ||
| 1H77Se HMQC | ||
| 1H77Se HMBC | ||
| 119Sn | 119Sn{1H} zgig | inverse gated |
| 1H119Sn HMQC | ||
| 1H119Sn HMBC | ||
| 119Sn{1H} inept-rd | ||
| 119Sn inept-pnd | Polarisation transfer | |
| 125Te | 125Te{1H} zgig | inverse gated |
| 1H125Te HMBC | ||
| 125Te{1H} inept-rd | Polarisation transfer | |
| 125Te inept-pnd | Polarisation transfer | |
| 195Pt | 195Pt{1H} | |
| 207Pb | 207Pb{1H} | |