Online resources and materials



Dr. Petra Bolte

Tel. +49 441 798 3478


W4 0-081 (Huygens)

W4-0-066 (Amira)


Online resources and materials

Guidelines how to report your microscopy data in publications:

  1. A guide to accurate reporting in digital image acquisition – can anyone replicate your microscopy data? Heddleston, J.M., Aaron, J.S., Khuon, S., Chew, T.-L. (2021)J. Cell Sci. doi: 10.1242/jcs.254144,(link)
  2. A guide to accurate reporting in digital image processing – can anyone reproduce your quantitative analysis? Aaron, J.S., Chew, T.-L. (2021) J. Cell Sci. doi:10.1242/jcs.254151,(link)


  1. Handbook of Biological Confocal Microscopy 
  2. Bioimage Data Analysis
  3. Fundamentals of Light Microscopy and Electronic Imaging

Tutorials and guides on microscopy for beginners and advanced users:

  1. "Tutorial: guidance for quantitative confocal microscopy", Nature Protocols volume 15, pages1585–1611(2020).  DOI: 10.1038/s41596-020-0313-9
  2. "A beginner’s guide to rigor and reproducibility in fluorescence imaging experiments", Molecular Biology of the CellVol. 29, No. 
  3. Neubias getaway 
  4. Intro videos of OiVM - Optical Imaging & Vital Microscopy

Online courses:

  1. List of online courses:
  2. Microcourses: background knowledge
  3. Bioimage analysis course Lecture BioImage Analysis 2020

Image analysis:

  1. Guide through the bio-image analysissoftware universe
  2. Neubias Academy
  3. Youtube channel of Neubias with webinars
  4. Amira online hands-on
  5. Huygens webinars
  6. Image analysis forum, where you can ask disturbing questions:
  7. Reproducible image handling and analysis Kota Miura and Simon F Nørrelykke.

Microscopy forums:


Fluorophores list:

  1. Fluorophores list
  2. Another Fluorophores list
  3. List of dyes and filter match (enable flash player)

Online materials provided by microscope companies

  1. Nikon
  2. Olympus
  3. Leica
  4. Zeiss
(Stand: 29.05.2024)  | 
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