Olympus FV 3000 Confocal and FLIM system
Contacts
Scientific director
Acknowledgement in Publications
This system was funded by the DFG, project number is 437530384.
According to new DFG rules since 2020 it is mandatory to acknowledge this funding in any publication.
For example as:
``The confocal microscopy system (FV3000) and the FLIM upgrade (Picoquant) were funded by the DFG, project number 437530384.´´
Olympus FV 3000 Confocal and FLIM system
The system is a standard inverted laser scanning confocal microscope with FLIM unit (fluorescence life time)
Excitation:
Confocal: The system is equipped with 7 cw diode lasers: 405nm, 445nm, 488nm, 514nm, 561nm , 594nm and 640nm.
FLIM: The life time imaging is possible with 443nm, 479nm, 559nm and 636nm lasers.
Detection:
Confocal: Emission wavelength collection is flexible because of the TruSpectral detector system. The system is equipped with four Cooled Gallium Arsenide Phosphide (GaAsP) PMT detectors to maximize detection efficiency.
FLIM: The system is equipped with 4 detectors: two hybrid detectors sensitive in the range 400-720nm and two SPAD (single-photon avalanche diode) detectors covering 400-1020nm range.
Objectives: 1.25x NA 0.04, 4x NA 0.16, 20x NA 0.8, 40x NA 1.4 oil, 30x NA 1.05 Silicon oil and 60x NA 1.3 Silicon oil. Silicon oil objectives are suitable for live cell imaging because of the refractive index match.
Further features: The system features eg. multiple ROI selection, Macro-to-Micro Imaging, resonant scanner with scan speeds of up to 438 frames/s (512 x 512 pixels), xyz, xzy, xyzt, xzyt, and lambda scan modes (both excitation and emission).
FLIM additional units allows to perform:
- Fluorescent life time (FLIM) measurements (Picoquant tutorial)
- FLIM-FRET (Förster resonance energy transfer) measurements (Picoquant tutorial)
- Anysotropy measurements (Picoquant anisotropy data analysis)
- Fluorescence correlation spectroscopy (FCS) measurements (Picoquant tutorial)
To use the microscope you will need three trainings:
- lab safety training (S2) performed by Dr. Nicola Brand
- Laser safety by Petra Bolte
- Microscope training by Petra Bolte
Also users will need to bring their own lab coats to access the lab.
Samples should be the level S1 (no viruses and no infections).
The lab safety training is performed on a regular basis twice a year. Important: Training and use of the FV3000 (the only microscope for FLIM/FRET/FRAP/FCS or Live Cell Imaging) is only possible with S2 instruction. An S2 instruction will take place on Thu, 23.03.2023 at 17:15 h online by Nicola Brandt. Please contact Petra Bolte or Nicola Brand to get the link. There will not be another date for this until next fall!