Leica SP5 Confocal/STED
Leica SP5 microscope combines possilibty to perform Stimulated emission depletion microscopy (STED) and fluorescent confocal microscopy.
Flexible excitation (470-700 nm), filter-free spectral detection, simultaneous 5-colour detection, Super-resolution STED at 592 nm (green dyes), resonant scanners, gated detection.
Excitation: The system is equipped with a white light laser (WLL) for a flexible choice of up to eight simultaneous excitation wavelengths (470-700 nm). The system further has a choice for argon laser excitation at 458 nm, 476 nm, 488 nm, 496 nm, and 514 nm.
Detection: Emission wavelength collection is flexible because of the filter-free spectral detection system. The system is equipped with three point detectors; three PMT detectors and two hybrid (HyD) detectors. The HyD detectors have gated detection.
Objectives: 10X/0.3 dry, 20X/0.75 IMM (multi immersion: for water, glycerol, and oil), 40X/1.3 oil, 63X/1.2 water, 100X/1.4 oil.
Further features: The system features eg. multiple ROI selection, scan speeds up to 7 frames/s (512x512 pixels), resonant scanner (8 kHz) with scan speeds of up to 28 frames/s (512 x 512 pixels), xyz, xzy, xyzt, xzyt, and lambda scan modes (both excitation and emission).
STED: The STED (STimulated Emission Depletion) systems is used with the above confocal system. Depletion: The system is equipped with a 592 nm continuous wave (CW) STED laser allowing for super-resolution microscopy. Sub-diffraction limited spatial (xy) resolution of down to ~50 nm standard green emitting STED dyes (eg. alexa488, Atto-488, abberior star 488, chromeo488). Objective: 100X/1.4.
Software for data analysis can be found here Leica LAS X