Leica SP5 Confocal/STED

Contacts

Scientific director

Prof. Dr. Henrik Mouritsen

Superusers

Dr. Petra Bolte

Tel. +49 441 798 3478

Apl. Prof. Dr. Karin Dedek

Tel. +49 441 798 3425

General email
 

W4 0-081

 

Leica SP5 Confocal/STED

Leica SP5 microscope combines possilibty to perform Stimulated emission depletion microscopy (STED) and fluorescent confocal microscopy.

Special features:
Flexible excitation (470-700 nm), filter-free spectral detection, simultaneous 5-colour detection, Super-resolution STED at 592 nm (green dyes), resonant scanners, gated detection.

The system is a standard inverted laser scanning confocal microscope. Leica-SP5-STED

Excitation: The system is equipped with a white light laser (WLL) for a flexible choice of up to eight simultaneous excitation wavelengths (470-700 nm). The system further has a choice for argon laser excitation at 458 nm, 476 nm, 488 nm, 496 nm, and 514 nm. 

Detection: Emission wavelength collection is flexible because of the filter-free spectral detection system. The system is equipped with three point detectors; three PMT detectors and two hybrid (HyD) detectors. The HyD detectors have gated detection. 

Objectives: 10X/0.3 dry, 20X/0.75 IMM  (multi immersion: for water, glycerol, and oil), 40X/1.3 oil, 63X/1.2 water, 100X/1.4 oil.

Further features: The system features eg. multiple ROI selection, scan speeds up to 7 frames/s (512x512 pixels), resonant scanner (8 kHz) with scan speeds of up to 28 frames/s (512 x 512 pixels), xyz, xzy, xyzt, xzyt, and lambda scan modes (both excitation and emission).

STED: The STED (STimulated Emission Depletion) systems is used with the above confocal system. Depletion: The system is equipped with a 592 nm continuous wave (CW) STED laser allowing for super-resolution microscopy. Sub-diffraction limited spatial (xy) resolution of down to ~50 nm standard green emitting STED dyes (eg. alexa488, Atto-488, abberior star 488, chromeo488). Objective: 100X/1.4.

Manual for the microscope usage. 

Software for data analysis can be found here Leica LAS X

 

(Stand: 21.08.2020)